Primer3

Primer3はPCR用のプライマーの設計支援ソフトウェア。オープンソースで開発されており、Webブラウザ上で、様々なパラメーターを考慮しながらプライマーの設計ができる。


Primer3でデザインし、実験で良好な結果を得たReal time RT-PCR用プライマー

• AMH

PRIMER PICKING RESULTS FOR AMH

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq 

LEFT PRIMER         18   22   61.53   54.55  4.00  0.00 TATCACTGGGGAGGGAGATAGG
RIGHT PRIMER       165   20   62.03   55.00  2.00  0.00 GTGGAAGGTGGGGTGTTTGT
SEQUENCE SIZE: 2008
INCLUDED REGION SIZE: 2008

PRODUCT SIZE: 148, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

• INHBB

PRIMER PICKING RESULTS FOR INHBB

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq 

LEFT PRIMER       2091   20   61.53   55.00  2.00  0.00 GTCATTCTGTTGGGCTGTGG
RIGHT PRIMER      2187   20   62.07   55.00  2.00  0.00 GGGTTCTCGCTCTGGTTTTG
SEQUENCE SIZE: 3516
INCLUDED REGION SIZE: 3516

PRODUCT SIZE: 97, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00

• AKT1

PRIMER PICKING RESULTS FOR AKT1

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq 

LEFT PRIMER        441   20   62.31   55.00  4.00  0.00 CACTGTCATCGAACGCACCT
RIGHT PRIMER       551   22   62.08   54.55  2.00  0.00 ATCTCCTCCTCCTCCTGCTTCT
SEQUENCE SIZE: 2610
INCLUDED REGION SIZE: 2610

PRODUCT SIZE: 111, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

• CTNNB1

PRIMER PICKING RESULTS FOR CTNNB1

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq 

LEFT PRIMER       3506   25   60.49   48.00  2.00  0.00 10.00 GGGTAGGGTAAATCAGTAAGAGGTG
RIGHT PRIMER      3610   22   61.06   45.45  2.00  0.00 10.00 AAGCATCGTATCACAGCAGGTT
SEQUENCE SIZE: 3697
INCLUDED REGION SIZE: 3697

PRODUCT SIZE: 105, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

• GSK3B

PRIMER PICKING RESULTS FOR GSK3B

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq 

LEFT PRIMER        948   21   61.13   52.38  2.00  0.00 12.00 ATGTATGGTCTGCTGGCTGTG
RIGHT PRIMER      1080   22   61.66   45.45  3.00  0.00 11.00 TCTCTGATTTGCTCCCTTGTTG
SEQUENCE SIZE: 1639
INCLUDED REGION SIZE: 1639

PRODUCT SIZE: 133, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00

• PTEN

PRIMER PICKING RESULTS FOR PTEN

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq 

LEFT PRIMER       1297   24   62.91   45.83  3.00  0.00 12.00 CTTTTGAAGACCATAACCCACCAC
RIGHT PRIMER      1433   23   61.95   47.83  2.00  0.00 10.00 CATTACACCAGTTCGTCCCTTTC
SEQUENCE SIZE: 3413
INCLUDED REGION SIZE: 3413

PRODUCT SIZE: 137, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00